Expression pattern of 5′-nucleotidase in Dictyostelium

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چکیده

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Expression pattern of 5′-nucleotidase in Dictyostelium

In order to analyze the expression pattern of the 5'-nucleotidase (5nt) gene in Dictyostelium, we made a fusion construct in which the 5nt promoter directed the expression of beta-galactosidase gene. The reporter gene was not active in vegetative amoebae but was expressed during the aggregation stage. At the slug stage, 5nt was highly expressed in pstAB cells. As the slug moved along the substr...

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Electrophoretic fractionation of 5'-nucleotidase.

Human isonucleotidases were separated by electrophoresis on a cellulose acetate membrane. Three 5'-nucleotidase forms, NTP1, NTP2, and NTP3, were resolved with this method and quantified by densitometry. The procedure was not only simple and rapid but also sufficiently precise (between-run CV < 20%) and sensitive (detected nucleotidase fractions of > 0.5 U/L). The effects of various treatments ...

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ژورنال

عنوان ژورنال: Mechanisms of Development

سال: 2002

ISSN: 0925-4773

DOI: 10.1016/s0925-4773(01)00580-9